Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pUPD2
  (Search Vector Database)
• Backbone manufacturer
  GoldenBraid kit from Diego Orzaez (Addgene kit # 1000000076 ), IBMCP, Valencia, Spain
• Backbone sizew/o insert(bp)2100
• Vector type
  Plant Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s)
  Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  Top10
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Infectious clone of Tobacco mosaic virus, 5' part
• Alt name
  TMV
• Alt name
  viral expression vector
• Species
  Synthetic
• Insert Size (bp)
  6622
• Mutation
  BsaI and BsmBI sites removed

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteBsmBI(destroyed during cloning)
• 3′ cloning siteBsmBI(destroyed during cloning)
• 5′ sequencing primerCCCGATCAACTCGAGTGCCA
• 3′ sequencing primerGAGGAAGCCTGCATAACG
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • pupd2-35s-tmvrepmp.gb
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry