Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pGEM T-easy
  (Search Vector Database)
• Backbone manufacturer
  Promega
• Backbone sizew/o insert(bp)3017
• Total vector size (bp)3620
• Vector type
  Plant Expression, CRISPR, Synthetic Biology ; Subcloning

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  U6-26p::sgRNA scaffold
• gRNA/shRNA sequence
  can be inserted via BbsI sites
• Species
  A. thaliana (mustard weed), Synthetic

Resource Information

• Supplemental Documents
  • pFH6 new genebank.gb
  • Cloning Protocol for pFH6_new and pUB-Cas9
• A portion of this plasmid was derived from a plasmid made by
  Peter Hegemann (HU Berlin)
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry

Depositor Comments