Ordering

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Backbone

• Vector backbone
  pRetrX-TRE3G
  (Search Vector Database)
• Backbone manufacturer
  Clontech
• Backbone sizew/o insert(bp)6600
• Total vector size (bp)8510
• Vector type
  Mammalian Expression, Retroviral ; Tet-On retrovirus
• Selectable markers
  Puromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  human MLKL NB(1-182)-2xFv-Venus
• Alt name
  oligomerizable MLKL N-terminal Bundle Brace
• Species
  H. sapiens (human)
• Insert Size (bp)
  1948
• Mutation
  amino acids 1-182
• GenBank ID
  NM_152649.3
• Entrez Gene
  MLKL(a.k.a. hMLKL)
• PromoterDox-inducible
• Tag/ Fusion Protein
  • Venus (C terminal on insert)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteClaI(not destroyed)
• 3′ cloning siteEcoRI(not destroyed)
• 5′ sequencing primerGGGCCCACCATGGTGAGCAAGGGCGAG
• 3′ sequencing primerGAATTCTTACTTGTACAGCTCGTC
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • PF4349
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Tet Systems Limited Use Label License
• Industry Terms
  • Not Available to Industry