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Backbone

• Vector backbone
  pAAV-EF1a-Flpo
  (Search Vector Database)
• Backbone manufacturer
  Karl Deisseroth
• Backbone sizew/o insert(bp)6109
• Total vector size (bp)7465
• Modifications to backbone
  The post-transcriptional regulatory WPRE was replaced by WPRE3. The hGH polyA sequence was replaced bySV40pA.
• Vector type
  Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  synaptophysin-GCaMP6s
• Alt name
  synaptophysin-GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
• Species
  R. norvegicus (rat); A. victoria (jellyfish)
• Insert Size (bp)
  1274
• GenBank ID
  24804
• PromoterEf1a, Human elongation factor-1 alpha

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteAscI(not destroyed)
• 3′ cloning siteRsrII(destroyed during cloning)
• 5′ sequencing primeracttctctccaaggtttgtc
• 3′ sequencing primerttgatatcgaattcataact
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • Restriction Digest - SmaI
  • AAV_Ef1a_DIO_SynGCaMP6s_WPRE3_SV40_.gb
• A portion of this plasmid was derived from a plasmid made by
  GCaMP6s was synthesized de novo based on sequences published by Douglas Kim et al, Janelia Research Campus in Chen et al, 2013 PMID: 23868258.The Synaptophysin-GCaMP6s fusion sequence was previously published by Ofer Yizhar's lab in Mahn et al, 2016 PMID: 26950004
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • genOway Notice of RIghts
• Industry Terms
  • Not Available to Industry