Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252
  (Search Vector Database)
• Backbone manufacturer
  Didier Trono
• Backbone sizew/o insert(bp)6857
• Total vector size (bp)9312
• Modifications to backbone
  PGK GFP removed (EcoRV and BamHI)and replaced with a multiple cloning site.WS1.6-WASp cassette cloned into MluI-SpeI site of new MCS.
• Vector type
  Lentiviral

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  Low Copy

Gene/Insert

• Gene/Insert name
  WASp
• Alt name
  Wiskott-Aldrich Syndrome Protein
• Alt name
  WAS
• Species
  H. sapiens (human)
• Insert Size (bp)
  1509
• Entrez Gene
  WAS(a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
• PromoterWS1.6

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteMluI(not destroyed)
• 3′ cloning siteSpeI(not destroyed)
• 5′ sequencing primergatcacgagactagcctcgag
• 3′ sequencing primercaacgggccacaactcctc
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  Backbone is from Addgene plasmid 12252 (Didier Trono)
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry