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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Addgene/pLVX-Puro-mRuby-Sec23A/1unit/36158
产品编号:36158
市  场 价:¥1500.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$75.00
品      牌: Addgene
公      司:Addgene
公司分类:
Addgene/pLVX-Puro-mRuby-Sec23A/1unit/36158
商品介绍

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid36158Standard format: Plasmid sent in bacteria as agar stab1$75
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-Puro
    (Search Vector Database)
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec23A
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEC23A(a.k.a. CLSD, hSec23A)
  • PromoterCMV
  • Tag/ Fusion Protein
    • mRuby (N terminal on insert)

Cloning Information

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteSal1(destroyed during cloning)
  • 3′ cloning siteXba1(not destroyed)
  • 5′ sequencing primerCMV
  • 3′ sequencing primerunknown
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
    • Seqbuilder file of the sequence
  • A portion of this plasmid was derived from a plasmid made by
    mRuby was synthesized to our requirements by Eurofins MWG
  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
    • 2 References

Depositor Comments

The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usageadaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript IISK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplifiedby PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)and cloned into pLVX-Puro–mRuby.

品牌介绍

Addgene正在为我们收集的200多个质粒提供克隆级DNA(cgDNA)。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供,我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤,从而缩短了实验时间。

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