Ordering

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Backbone

• Vector backbone
  pTY
  (Search Vector Database)
• Backbone manufacturer
  LJ Chang
• Backbone sizew/o insert(bp)8701
• Total vector size (bp)10927
• Modifications to backbone
  Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
• Vector type
  Lentiviral

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

Gene/Insert1

• Gene/Insert name
  IRBP156-CHER
• Alt name
  interphotoreceptor retinoid-binding protein
• Alt name
  mCherry
• Species
  M. musculus (mouse), Synthetic
• Insert Size (bp)
  1128
• Promotermurine interphotoreceptor retinoid-binding protein 156 (pos 2524-2873)

Cloning Informationfor Gene/Insert 1

• Cloning methodUnknown
• 5′ sequencing primerN/A
• 3′ sequencing primerN/A
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  RK-GFP
• Alt name
  human rhodopsin kinase
• Alt name
  GFP
• Species
  H. sapiens (human), Synthetic
• Insert Size (bp)
  1027
• Promoterhuman rhodopsin kinase (pos 3723-4019)

Cloning Informationfor Gene/Insert 2

• Cloning methodUnknown
• 5′ sequencing primerN/AN
• 3′ sequencing primerN/A
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  The IRBP promoter was amplified from BP1783-EGFP kindly provided by Jeffrey Boatright, Emory University.
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms
  • Not Available to Industry