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Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pAAV-EF1a-FLEX-GTB
  (Search Vector Database)
• Backbone manufacturer
  Callaway Addgene 26197
• Total vector size (bp)8022
• Modifications to backbone
  Replaced GFP-TVA in pAAV-EF1a-FLEX-GTB with Cerulean
• Vector type
  Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Cerulean-E2A-RG
• Alt name
  rabies virus glycoprotein
• Species
  rabies virus
• Insert Size (bp)
  2367
• PromoterEF1a
• Tag/ Fusion Protein
  • cerulean (N terminal on insert)

Cloning Information

• Cloning methodLigation Independent Cloning
• 5′ sequencing primeratttgccctttttgagtttgg
• 3′ sequencing primerCAAGGCTGGTGGGCACTGG
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  The original plasmid is Addgene Plasmid #26197,
• Terms and Licenses
  • UBMTA
  • genOway Notice of RIghts
  • Vanderbilt-Cerulean
• Industry Terms
  • Not Available to Industry
• Articles Citing this Plasmid
  • 2 References

Depositor Comments