Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  psiCHECK-2
  (Search Vector Database)
• Backbone manufacturer
  Promega
• Backbone sizew/o insert(bp)6273
• Total vector size (bp)6302
• Vector type
  Mammalian Expression, Luciferase ; microRNA activity

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  fully complimentary miR-34 target site
• Species
  Synthetic
• Insert Size (bp)
  29

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteXhoI(not destroyed)
• 3′ cloning siteNotI(not destroyed)
• 5′ sequencing primerCGTGCTGAAGAACGAGCAGT
• 3′ sequencing primerCAAACCCCCGCCTCCACGG
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms
  • Not Available to Industry
• Article Citing this Plasmid
  • 1 Reference

Depositor Comments