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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Addgene/pHL1668/1unit/37633
产品编号:37633
市  场 价:¥1500.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$75.00
品      牌: Addgene
公      司:Addgene
公司分类:
Addgene/pHL1668/1unit/37633
商品介绍

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid37633Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZ with ColE origin
    (Search Vector Database)
  • Backbone manufacturer
    Lim Lab
  • Modifications to backbone
    Plasmid modified from Rolf Lutz and Hermann Bujard, 1997
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert1

  • Gene/Insert name
    GFP
  • Alt name
    green fluorescent protein
  • Insert Size (bp)
    717
  • PromoterpConM12NoHind
  • Tags/ Fusion Proteins
    • ASV degradation tag (C terminal on backbone)
    • glgC leader region (N terminal on backbone)

Cloning Informationfor Gene/Insert 1

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteSphI(not destroyed)
  • 3′ cloning siteNheI(not destroyed)
  • 5′ sequencing primerCTCATGA GCGGATACAT ATTTGAA
  • 3′ sequencing primercgcggatcc AAGGCCATCCGTCAGGATGGCCTT
  • (Common Sequencing Primers)

Gene/Insert2

  • Gene/Insert name
    TetR
  • PromoterpConNoHind

Cloning Informationfor Gene/Insert 2

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteXmaI(not destroyed)
  • 3′ cloning siteHindIII(not destroyed)
  • 5′ sequencing primerGCTGGGATTA CACATGGCAT GGAT
  • 3′ sequencing primerccggaattc ACTGCTCACA AGAAAAAAGG CACG
  • (Common Sequencing Primers)

Gene/Insert3

  • Gene/Insert name
    CsrA
  • Insert Size (bp)
    186
  • Entrez Gene
    csrA(a.k.a. b2696, ECK2691, zfiA)
  • PromoterpConNoHind

Cloning Informationfor Gene/Insert 3

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteNotI(not destroyed)
  • 3′ cloning siteApaI(not destroyed)
  • 5′ sequencing primerggccaag CTT TAATC GTACAGGGTA GTACAAATA
  • 3′ sequencing primerAGCTGATACC GCTCGCCGCA GCCGAACG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
    • Ancillary Agreement for Plasmids Containing FP Materials
  • Industry Terms
    • Not Available to Industry
品牌介绍

Addgene正在为我们收集的200多个质粒提供克隆级DNA(cgDNA)。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供,我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤,从而缩短了实验时间。

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