Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pZ with p15a origin
  (Search Vector Database)
• Backbone manufacturer
  Lim Lab
• Modifications to backbone
  Plasmid modified from Rolf Lutz and Hermann Bujard, 1997
• Vector type
  Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s)
  Kanamycin
• Growth Temperature
  37°C
• Growth Strain(s)
  XL1 Blue
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  CsrA
• Species
  E. coli
• Insert Size (bp)
  186
• Entrez Gene
  csrA(a.k.a. b2696, ECK2691, zfiA)
• PromoterpLtetO-1

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteXmaI(not destroyed)
• 3′ cloning siteHindIII(not destroyed)
• 5′ sequencing primerCAGTCATAGCCGAATAGCCT
• 3′ sequencing primerccggaattc ACTGCTCACA AGAAAAAAGG CACG
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry