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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Addgene/pWZL Neo Myr Flag ACVR1/1unit/20412
产品编号:20412
市  场 价:¥1500.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$75.00
品      牌: Addgene
公      司:Addgene
公司分类:
Addgene/pWZL Neo Myr Flag ACVR1/1unit/20412
商品介绍

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid20412Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWZL-Neo-Myr-Flag-DEST
    (Search Vector Database)
  • Backbone manufacturer
    William Hahn Lab (available at Addgene, #15300)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3 cells to prevent recombination.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ACVR1
  • Alt name
    activin A receptor, type I
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001105
  • Entrez Gene
    ACVR1(a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
  • Tags/ Fusion Proteins
    • Myr (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning methodGateway Cloning
  • 5′ sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)
  • 3′ sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    2-B4
  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
    • 1 Reference

Depositor Comments

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations.Please see the following link for plasmids from this article that are not part of the kinase libraryhttp://www.addgene.org/pubmed/17574021

品牌介绍

Addgene正在为我们收集的200多个质粒提供克隆级DNA(cgDNA)。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供,我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤,从而缩短了实验时间。

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