Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pBlueScript II KS(+)
  (Search Vector Database)
• Backbone manufacturer
  Stratagene
• Backbone sizew/o insert(bp)5600
• Vector type
  Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s)
  Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  p21 promoter deletion mutant
• Alt name
  p21, WAF1
• Species
  H. sapiens (human)
• Insert Size (bp)
  1497
• Entrez Gene
  CDKN1A(a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
• Tag/ Fusion Protein
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ sequencing primerna
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms
  • Not Available to Industry
• Article Citing this Plasmid
  • 1 Reference