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Backbone

• Vector backbone
  pL1A0*B0*_Leu
  (Search Vector Database)
• Backbone sizew/o insert(bp)4885
• Total vector size (bp)11678
• Vector type
  Yeast Expression, Cre/Lox, Synthetic Biology
• Selectable markers
  LEU2

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert1

• Gene/Insert name
  Phytochrome B
• Alt name
  PhyB
• Species
  A. thaliana (mustard weed)
• Insert Size (bp)
  1863
• Mutation
  AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
• Entrez Gene
  PHYB(a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
• PromoterTDH3
• Tags/ Fusion Proteins
  • Cdc1 NLS (C terminal on insert)
  • CreN (C terminal on insert)

Cloning Informationfor Gene/Insert 1

• Cloning methodGibson Cloning
• 5′ sequencing primerTTGGTCGGCTCTTTTCTT
• 3′ sequencing primerCCCTGAAATTATTCCCCT
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  Phytochrome interacting factor 3
• Alt name
  PIF3
• Species
  A. thaliana (mustard weed)
• Insert Size (bp)
  1572
• Entrez Gene
  PIF3(a.k.a. AT1G09530, F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, phytochrome interacting factor 3, purple acid phosphatase 3)
• PromoterFBA1
• Tags/ Fusion Proteins
  • SV40 NLS (C terminal on insert)
  • CreC (C terminal on insert)

Cloning Informationfor Gene/Insert 2

• Cloning methodGibson Cloning
• 5′ sequencing primerTTCCTTCTTCTTCGCCCA
• 3′ sequencing primerAAGAAAAGAGCCGACCAA
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • pLH_Scr16.gbk
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry