Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pGL3
  (Search Vector Database)
• Backbone manufacturer
  Promega
• Vector type
  Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  RpR 150 enhancer and hsp70 minimal promoter
• Species
  D. melanogaster (fly)
• PromoterRpR 150 enhancer and hsp70 minimal promoter
• Tag/ Fusion Protein
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteKpnI(not destroyed)
• 3′ cloning siteHindIII(not destroyed)
• 5′ sequencing primerRVprimer3
• 3′ sequencing primerLucNrev
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms
  • Not Available to Industry