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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Addgene/pFYF1328 EGFP Site#3/1unit/47513
产品编号:47513
市  场 价:¥1500.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$75.00
品      牌: Addgene
公      司:Addgene
公司分类:
Addgene/pFYF1328 EGFP Site#3/1unit/47513
商品介绍

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid47513Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MLM3636
    (Search Vector Database)
  • Backbone manufacturer
    Joung lab (Addgene plasmid #43860)
  • Backbone sizew/o insert(bp)2278
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA-EGFP site 3
  • Species
    Synthetic
  • Insert Size (bp)
    20
  • PromoterU6

Cloning Information

  • Cloning methodRestriction Enzyme
  • 5′ cloning siteBsmBI(not destroyed)
  • 3′ cloning siteBsmBI(not destroyed)
  • 5′ sequencing primerhU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Target site: GGTGGTGCAGATGAACTTCA

gRNA expression vector targeting EGFP for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9)

For more information on Joung Lab CRISPR Plasmids please refer to:images/Addgene/

品牌介绍

Addgene正在为我们收集的200多个质粒提供克隆级DNA(cgDNA)。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供,我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤,从而缩短了实验时间。

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