Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pLenti pRRL-SV40(puro)_CMV(mcs)
  (Search Vector Database)
• Backbone manufacturer
  unknown
• Backbone sizew/o insert(bp)7711
• Total vector size (bp)9935
• Vector type
  Mammalian Expression, Lentiviral
• Selectable markers
  Puromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Top10
• Copy number
  Low Copy

Gene/Insert

• Gene/Insert name
  Erk activity reporter
• Species
  Synthetic
• Insert Size (bp)
  2224
• PromoterCMV
• Tag/ Fusion Protein
  • His-Myc (N terminal on insert)

Cloning Information

• Cloning methodLigation Independent Cloning
• 5′ sequencing primerCGACTCTAGAGGATCCGGAGATATACCATGG
• 3′ sequencing primerCTAGACTGCAGGATCCTGGTGATGGTGATGG
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  Karel Svoboda, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry
• Article Citing this Plasmid
  • 1 Reference

Depositor Comments