Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 80032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV-MCS(Search Vector Database)
- Backbone manufacturerStratagene
- Backbone size(bp)2892
- Modifications to backbonea NotI-NotI fragment containing pAAV-MCS backbone was used for the cloning of pAAV-2Aneo.
- Vector typeAAV, Cre/Lox ; a platform vector to construct targeting vectors by inserting 5' and 3' arms
- Selectable markersNeomycin (select with G418)
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ sequencing primerSK primer
- 3′ sequencing primerKS primer (Common Sequencing Primers)
Resource Information
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
Upon construction of a targeting vector with pAAV-2Aneo, the “frame adjuster” should be first cleaved with BspEI, MluI, or BsrGI and then self-religated so that 2Aneo is translated in frame with the 5’ portion of the targeted endogenous gene.This truncation of the frame adjuster should be done prior to the incorporation of 5' and 3' arms into pAAV-2Aneo, if arms carry restriction enzyme sites cleaved during the truncation of the frame adjuster (i.e., BspEI, MluI, or BsrGI sites).This plasmid and pAAV-2Aneo v2 differ only by the sequences of the frame adjusters.
A following article describes how to use pAAV-2Aneo to construct AAV-based targeting vectors: Karnan et al. Bio-Protoc, 6(24): e2058, 2016. (http://www.bio-protocol.org/e2058)
Addgene正在为我们收集的200多个质粒提供克隆级DNA（cgDNA）。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供，我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤，从而缩短了实验时间。
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