Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pWZL-Neo-Myr-Flag-DEST
  (Search Vector Database)
• Backbone manufacturer
  William Hahn Lab (available at Addgene, #15300)
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Growth instructions
  Stbl3 cells to prevent recombination.
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  AKT3
• Alt name
  v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
• Species
  H. sapiens (human)
• GenBank ID
  NM_005465
• Entrez Gene
  AKT3(a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)
• Tags/ Fusion Proteins
  • Myr (N terminal on backbone)
  • Flag (N terminal on backbone)

Cloning Information

• Cloning methodGateway Cloning
• 5′ sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)
• 3′ sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG)
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  2-F2
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry

Depositor Comments