Ordering

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Backbone

• Vector backbone
  phi-Yellow-Dest
  (Search Vector Database)
• Backbone manufacturer
  Evrogen
• Backbone sizew/o insert(bp)4000
• Total vector size (bp)9760
• Modifications to backbone
  Inserted dCas9-3xNLS-VP64 along with pPGK1_mKATE-HSVpolyA
• Vector type
  Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  dCas9_VP64 (human-codon-optimized)
• Species
  H. sapiens (human)
• Insert Size (bp)
  4410
• PromoterCMV
• Tag/ Fusion Protein
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning methodLigation Independent Cloning
• 5′ sequencing primerTCTTCGCTATTACGCCAGCCC
• 3′ sequencing primerAGTGCGGCGACGATAGTCATGCCCC
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • Genbank file
• Terms and Licenses
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms
  • Not Available to Industry
• Articles Citing this Plasmid
  • 3 References

Depositor Comments