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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Addgene/peft-3::cas-9::tbb-2 3'UTR/1unit/48960
产品编号:48960
市  场 价:¥1500.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$75.00
品      牌: Addgene
公      司:Addgene
公司分类:
Addgene/peft-3::cas-9::tbb-2 3'UTR/1unit/48960
商品介绍

Ordering

ItemCatalog #DescriptionQuantityPrice (USD)
Plasmid48960Standard format: Plasmid sent in bacteria as agar stab1$75
Add to Cart

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST
    (Search Vector Database)
  • Backbone manufacturer
    life technologies
  • Backbone sizew/o insert(bp)4959
  • Total vector size (bp)8732
  • Modifications to backbone
    a unc-54 3'UTR was cloned to the initial pDEST vector
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cas9
  • Promotereef-1A.1 (eft-3)

Cloning Information

  • Cloning methodGateway Cloning
  • 5′ sequencing primerTGCTCActccgtagcagcc
  • 3′ sequencing primeraagaaagaagagtgatagagaagaaggg
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
    • UBMTA
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
    • 2 References

Depositor Comments

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: images/Addgene/WBGene00001168

品牌介绍

Addgene正在为我们收集的200多个质粒提供克隆级DNA(cgDNA)。以这种格式可获得的质粒包括多种流行的质粒和具有高克隆潜力的主链。通过将这些质粒以cgDNA的形式提供,我们希望帮助希望从Addgene那里获得质粒后立即开始克隆的科学家-消除了当人们在细菌刺中接受质粒时所需的扩增和提取步骤,从而缩短了实验时间。

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